Oliveria decumbens Extract Exhibits Hepatoprotective Effects Against Bile Duct Ligation-Induced Liver Injury in Rats by Reducing Oxidative Stress

Background: Cholestasis is described as a disease in which bile flow from the liver reduced or stopped, and due to its oxidative effects, irreversible consequences may occur. Objectives: Due remarkable antioxidant properties of Oliveria decumbens (OD) contribution oxidants progression duct ligation (BDL)-induced cholestasis, this research aimed examine how OD ethanolic extract affected damage oxidant-antioxidant balance markers BDL-induced cholestasis. Methods: Forty male Wistar rats weighing 200 - 250 g were used. was induced using BDL approach. The categorized into four groups: Group 1, sham-control (SC); group 2, cholestatic; 3, SC + OD; 4, cholestatic OD. A dose administered orally (500 mg/kg/day) for seven days. Seven days following surgery, rats’ blood samples collected; after sacrifice, part tissue isolated. histopathological examination performed, while rest stored at -70°C liquid nitrogen. Heparin-containing tubes used gather samples. In plasma hepatic tissue, biochemical tests, evaluations, stress staining levels performed. Results: Our findings showed that could effectively reduce injury by reducing activity function enzymes (AST ALP). At same time, it did not affect total bilirubin protein. Bile ligation-induced protein oxidation (PCO) reactive nitrogen species (NO) significantly decreased OD, also promoted capacity enhancing superoxide dismutase (SOD) activities. Moreover, treatment prevented proliferative changes histopathologic analysis. Conclusions: study confirmed exerts substantial hepatoprotective activities against cholestasis improving regulating stress. It be beneficial therapeutic agent managing Bioassay-guided isolation identification bioactive secondary metabolites can further direct discovery potential natural-based drug candidates.